Journal article

Publication year: 1991

Pages: 1893-1902

Journal: The journal of general microbiology

Volume number: 137

Issue number: 8

ISSN: 0022-1287

DOI Link: https://doi.org/10.1099/00221287-137-8-1893

Publisher: Cambridge Univ. Press

Abstract: 

A frequency matrix of positive results for phena defined in a previous phenetic classification was constructed. A total of 329 physiological characters from 782 strains was taken as the basis for this identification matrix. The minimum number of diagnostic characters for the matrix was selected using computer programs for calculation of different separation indices (CHARSEP) and selection of group diagnostic properties (DIACHAR). The resulting matrix consisted of 52 phena versus 50 characters. Overlap of phena was found to be relatively small (OVERMAT program). Identification scores for the most typical hypothetical organism of each phenon was satisfactory (MOSTTYP program). The matrix was evaluated theoretically and practically (MATIDEN program). For members of major clusters and subclusters, e.g. Streptomyces albidoflavus, S. halstedii, S. griseus, S. fradiae, S. rimosus and S. albus, identification scores were high. Organisms of phena containing only small numbers of strains could be identified correctly, but with lower accuracy. The identification rate of the matrix (Willcox probability > 0.90) in the theoretical evaluation was 84.39%, and in the practical evaluation 78.12%.

Harvard Citation style: Kämpfer, P. and Kroppenstedt, R. (1991) Probabilistic identification of streptomycetes using miniaturized physiological tests, The journal of general microbiology, 137(8), pp. 1893-1902. https://doi.org/10.1099/00221287-137-8-1893

APA Citation style: Kämpfer, P., & Kroppenstedt, R. (1991). Probabilistic identification of streptomycetes using miniaturized physiological tests. The journal of general microbiology. 137(8), 1893-1902. https://doi.org/10.1099/00221287-137-8-1893