Journal article

Publication year: 1992

Pages: 524-528

Journal: Applied Microbiology and Biotechnology

Volume number: 37

Issue number: 4

ISSN: 0175-7598

DOI Link: https://doi.org/10.1007/BF00180981

Publisher: Springer

Abstract: 

The anaerobic degradation of phenol under denitrifying conditions by a bacterial consortium was studied both in batch and continuous cultures. Anaerobic degradation was dependent on NO_f3 ^p− and concentrations up to 4 mm phenol were degraded within 2–5 days. During continuous growth in a fermenter, steady states could be maintained at eight dilution rates (D) corresponding to residence times between 12.5 and 50 h. Culture wash-out occurred at D=0.084 h⁻¹. The kinetic parameters obtained for anaerobic degradation of phenol under denitrifying conditions by the consortium were: maximam specific growth rate = 0.091 h⁻¹; saturation constant = 4.91 mg phenol/l; true growth yield = 0.57 mg dry wt/mg phenol; maintenance coefficient = 0.013 mg phenol/mg dry wt per hour. The Haldane model inhibition constant was estimated from batch culture data giving a value of 101 mg/l. The requirement of CO₂ for the anaerobic degradation of phenol with NO_f3 ^p− indicates that phenol carboxylation to 4-hydroxybenzoate was the first step of phenol degradation by this culture. 4-Hydroxybenzoate, proposed as an intermediate of phenol carboxylation under these conditions, was detected only in continuous cultures at very low growth rates (D=0.02 h⁻¹), but was never detected as a free intermediary metabolite either in batch or in continuous cultures.

Harvard Citation style: Khoury, N., Dott, W. and Kämpfer, P. (1992) Anaerobic degradation of phenol in batch and continuous cultures by a denitrifying bacterial consortium, Applied Microbiology and Biotechnology, 37(4), pp. 524-528. https://doi.org/10.1007/BF00180981

APA Citation style: Khoury, N., Dott, W., & Kämpfer, P. (1992). Anaerobic degradation of phenol in batch and continuous cultures by a denitrifying bacterial consortium. Applied Microbiology and Biotechnology. 37(4), 524-528. https://doi.org/10.1007/BF00180981