Journal article

Publication year: 2017

Pages: 58-66

Journal: Journal of Immunological Methods

Volume number: 440

ISSN: 0022-1759

eISSN: 1872-7905

DOI Link: https://doi.org/10.1016/j.jim.2016.11.007

Publisher: Elsevier

Abstract: 

Strenuous exercise is followed by an elevation of many cytokines with inflammation regulating properties. Since most cytokines act at pico- or nanomolar concentrations many investigations failed to detect their concentrations in vivo. Hence, the aim of this study was to evaluate the significance of cytokine measurements (IL-1β, TNF-α, IL-1ra, IL-6, CCL2 and CXCL8) in a stimulated whole-blood culture (sWBC) compared to serum with respect to their exercise-induced kinetics and detection rates. 40 male volunteers (age: 25,5 ± 4,3 years, BMI: 24,00 ± 2,24, VO₂peak: 46,9 ± 4,1 mL/kg × min) performed 60 min of intensive bicycle exercise (80% VO₂peak). Blood samples were taken before and for up to 24 h after exercise. All cytokines were determined by a multiplex ELISA. There were weak to moderate correlations between cytokines in sWBC and serum. While exercise did not affect pro-inflammatory cytokines in serum, in sWBC only IL-1β was increased 1.2-fold at 3 h (p < 0,05). All other cytokines increased both in sWBC and serum. The detection rate was superior in sWBC vs serum for most cytokines. Exercise-induced cytokine kinetics in sWBC do not reflect systemic changes. Both approaches provide a synergistic insight into inflammatory processes on the cytokine level.

Harvard Citation style: Pilat, C., Krüger, K., Frech, T. and Mooren, F. (2017) Exercise-induced cytokine changes in antigen stimulated whole-blood cultures compared to serum, Journal of Immunological Methods, 440, pp. 58-66. https://doi.org/10.1016/j.jim.2016.11.007

APA Citation style: Pilat, C., Krüger, K., Frech, T., & Mooren, F. (2017). Exercise-induced cytokine changes in antigen stimulated whole-blood cultures compared to serum. Journal of Immunological Methods. 440, 58-66. https://doi.org/10.1016/j.jim.2016.11.007