Journal article

Publication year: 2011

Pages: 1385-1394

Journal: International Journal of Obesity

Volume number: 35

Issue number: 11

ISSN: 0307-0565

eISSN: 1476-5497

DOI Link: https://doi.org/10.1038/ijo.2010.283

Publisher: Springer Nature [academic journals on nature.com]

Abstract: 

Objective: We investigated the expression of protein tyrosine phosphatase-interacting protein 51 (PTPIP51) and its interaction with protein tyrosine phosphatase 1B (PTP1B) and 14-3-3 beta in mice exhibiting insulin resistance and obesity.

Design: A total of 20 mice were included in the study. Eight control animals were fed a normal standard diet, six animals were fed a high-fat diet and six animals were submitted to a treadmill training parallel to the feeding of a high-fat diet. After 10 weeks, a glucose tolerance test was performed and abdominal adipose tissue samples of the animals were collected.

Results: PTPIP51 protein was identified in the adipocytes of all samples. PTPIP51 interacted with PTP1B and with 14-3-3 beta protein. Compared with untrained mice fed a standard diet, the interaction of PTPIP51 with PTP1B was reduced in high-fat diet-fed animals. The highest interaction of PTPIP51 with 14-3-3 beta was seen in trained animals on high-fat diet, whereas untrained animals on high-fat diet displayed lowest values.

Conclusion: PTPIP51 is expressed in adipose tissue of humans, rats and mice. Obesity with enhanced insulin resistance resulted in a reduction of PTPIP51 levels in adipocytes and influenced the interactions with PTP1B and 14-3-3 beta. The interaction of PTPIP51 with PTP1B suggests a regulatory function of PTPIP51 in insulin receptor signal transduction. The interaction of PTPIP51 with 14-3-3 beta, especially in trained individuals, hints to an involvement of PTPIP51 in the downstream regulation of insulin action.

Harvard Citation style: Bobrich, M., Brobeil, A., Mooren, F., Krüger, K., Steger, K., Tag, C., et al. (2011) PTPIP51 interaction with PTP1B and 14-3-3β in adipose tissue of insulin-resistant mice, International Journal of Obesity, 35(11), pp. 1385-1394. https://doi.org/10.1038/ijo.2010.283

APA Citation style: Bobrich, M., Brobeil, A., Mooren, F., Krüger, K., Steger, K., Tag, C., & Wimmer, M. (2011). PTPIP51 interaction with PTP1B and 14-3-3β in adipose tissue of insulin-resistant mice. International Journal of Obesity. 35(11), 1385-1394. https://doi.org/10.1038/ijo.2010.283