Journal article
Authors list: Huang, Luyao; Min, Yao; Schiessl, Sarah; Xiong, Xinghua; Jan, Habib U.; He, Xin; Qian, Wei; Guan, Chunyun; Snowdon, Rod J.; Hua, Wei; Guan, Mei; Qian, Lunwen
Publication year: 2021
Journal: Plant Science: An international journal of experimental plant biology
Volume number: 310
ISSN: 0168-9452
eISSN: 1873-2259
DOI Link: https://doi.org/10.1016/j.plantsci.2021.110980
Publisher: Elsevier
Abstract:
Flowering is an important turning point from vegetative growth to reproductive growth, and vernalization is an essential condition for the flowering of annual winter plants. To investigate the genetic architecture of flowering time in rapeseed, we used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with flowering time in 203 Chinese semi-winter rapeseed inbred lines. Twenty-one haplotype regions carrying one or more candidate genes showed a significant association with flowering time. Interestingly, we detected a SNP (Bn-scaff_22728_1-p285715) located in exon 3 of the BnVIN3-C03 gene that showed a significant association with flowering time on chromosome C03. Based on the SNP alleles A and G, two groups of accessions with early and late flowering time phenotypes were selected, respectively, and PCR amplification and gene expression analysis were combined to reveal the structural variation of the BnVIN3-C03 gene that affected flowering time. Moreover, we found that BnVIN3-C03 inhibited the expression of BnFLC-A02, BnFLC-A03.1, BnFLC-A10 and BnFLC-C03.1, thus modulating the flowering time of Brassica napus. This result provides insight into the genetic improvement of flowering time in B. napus.
Citation Styles
Harvard Citation style: Huang, L., Min, Y., Schiessl, S., Xiong, X., Jan, H., He, X., et al. (2021) Integrative analysis of GWAS and transcriptome to reveal novel loci regulation flowering time in semi-winter rapeseed, Plant Science: An international journal of experimental plant biology, 310, Article 110980. https://doi.org/10.1016/j.plantsci.2021.110980
APA Citation style: Huang, L., Min, Y., Schiessl, S., Xiong, X., Jan, H., He, X., Qian, W., Guan, C., Snowdon, R., Hua, W., Guan, M., & Qian, L. (2021). Integrative analysis of GWAS and transcriptome to reveal novel loci regulation flowering time in semi-winter rapeseed. Plant Science: An international journal of experimental plant biology. 310, Article 110980. https://doi.org/10.1016/j.plantsci.2021.110980
