Journal article

Publication year: 2021

Journal: Molecular Breeding

Volume number: 41

Issue number: 12

ISSN: 1380-3743

eISSN: 1572-9788

Open access status: Hybrid

DOI Link: https://doi.org/10.1007/s11032-021-01270-9

Publisher: Springer

Abstract: 
Barley mild mosaic virus (BaMMV), transmitted by the soil-borne protist Polymyxa graminis, has a serious impact on winter barley production. Previously, the BaMMV resistance gene rym15 was mapped on chromosome 6HS, but the order of flanking markers was non-collinear between different maps. To resolve the position of the flanking markers and to enable map-based cloning of rym15, two medium-resolution mapping populations Igri (susceptible) x Chikurin Ibaraki 1 (resistant) (IxC) and Chikurin Ibaraki 1 x Uschi (susceptible) (C x U), consisting of 342 and 180 F-2 plants, respectively, were developed. Efficiency of the mechanical inoculation of susceptible standards varied from 87.5 to 100% and in F-2 populations from 90.56 to 93.23%. Pheno-typing of F-2 plants and corresponding F-3 families revealed segregation ratios of 250 s:92r (I x C, chi(2) =0.659) and 140 s:40r (C xU, chi(2) =0.741), suggesting the presence of a single recessive resistance gene. After screening the parents with the 50 K Infinium chip and anchoring corresponding SNPs to the barley reference genome, 8 KASP assays were developed and used to remap the gene. Newly constructed maps revealed a collinear order of markers, thereby allowing the identification of high throughput flanking markers. This study demonstrates how construction of medium-resolution mapping populations in combination with robust phenotyping can efficiently resolve conflicting marker ordering and reduce the size of the target interval. In the reference genome era and genome-wide genotyping era, medium-resolution mapping will help accelerate candidate gene identification for traits where phenotyping is difficult.

Harvard Citation style: Wang, Y., Habekuss, A., Snowdon, R., Ordon, F. and Perovic, D. (2021) Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping, Molecular Breeding, 41(12), Article 76. https://doi.org/10.1007/s11032-021-01270-9

APA Citation style: Wang, Y., Habekuss, A., Snowdon, R., Ordon, F., & Perovic, D. (2021). Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping. Molecular Breeding. 41(12), Article 76. https://doi.org/10.1007/s11032-021-01270-9