Journal article
Authors list: Böhringer, Nils; Kramer, Jil-Christine; de la Mora, Eugenio; Padva, Leo; Wuisan, Zerlina G.; Liu, Yang; Kurz, Michael; Marner, Michael; Nguyen, Hai; Amara, Patricia; Yokoyama, Kenichi; Nicolet, Yvain; Mettal, Ute; Schäberle, Till F.
Publication year: 2023
Pages: 943-952.e7
Journal: Cell Chemical Biology
Volume number: 30
Issue number: 8
ISSN: 2451-9456
eISSN: 2451-9448
Open access status: Bronze
DOI Link: https://doi.org/10.1016/j.chembiol.2023.06.011
Publisher: Cell Press
Abstract:
Darobactins represent a class of ribosomally synthesized and post-translationally modified peptide (RiPP) antibiotics featuring a rare bicyclic structure. They target the Bam-complex of Gram-negative bacteria and exhibit in vivo activity against drug-resistant pathogens. First isolated from Photorhabdus species, the cor-responding biosynthetic gene clusters (BGCs) are widespread among g-proteobacteria, including the genera Vibrio, Yersinia, and Pseudoalteromonas (P.). While the organization of the BGC core is highly conserved, a small subset of Pseudoalteromonas carries an extended BGC with additional genes. Here, we report the identification of brominated and dehydrated darobactin derivatives from P. luteoviolacea strains. The marine derivatives are active against multidrug-resistant (MDR) Gram-negative bacteria and showed solubility and plasma protein binding ability different from darobactin A, rendering it more active than darobactin A. The halogenation reaction is catalyzed by DarH, a new class of flavin-dependent halogenases with a novel fold.
Citation Styles
Harvard Citation style: Böhringer, N., Kramer, J., de la Mora, E., Padva, L., Wuisan, Z., Liu, Y., et al. (2023) Genome- and metabolome-guided discovery of marine BamA inhibitors revealed a dedicated darobactin halogenase, Cell chemical biology, 30(8), pp. 943-952.e7. https://doi.org/10.1016/j.chembiol.2023.06.011
APA Citation style: Böhringer, N., Kramer, J., de la Mora, E., Padva, L., Wuisan, Z., Liu, Y., Kurz, M., Marner, M., Nguyen, H., Amara, P., Yokoyama, K., Nicolet, Y., Mettal, U., & Schäberle, T. (2023). Genome- and metabolome-guided discovery of marine BamA inhibitors revealed a dedicated darobactin halogenase. Cell chemical biology. 30(8), 943-952.e7. https://doi.org/10.1016/j.chembiol.2023.06.011