Journal article

Direct readout of protein-protein interactions by mass spectrometry from protein-DNA microarrays


Authors listBecker, CFW; Wacker, R; Bouschen, W; Seidel, R; Kolaric, B; Lang, P; Schroeder, H; Müller, O; Niemeyer, CM; Spengler, B; Goody, RS; Engelhard, M

Publication year2005

Pages7635-7639

JournalAngewandte Chemie International Edition

Volume number44

Issue number46

ISSN1433-7851

DOI Linkhttps://doi.org/10.1002/anie.200502908

PublisherWiley


Abstract

Catching proteins: Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA‐directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates. Catching proteins: Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA‐directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates.




Citation Styles

Harvard Citation styleBecker, C., Wacker, R., Bouschen, W., Seidel, R., Kolaric, B., Lang, P., et al. (2005) Direct readout of protein-protein interactions by mass spectrometry from protein-DNA microarrays, Angewandte Chemie International Edition, 44(46), pp. 7635-7639. https://doi.org/10.1002/anie.200502908

APA Citation styleBecker, C., Wacker, R., Bouschen, W., Seidel, R., Kolaric, B., Lang, P., Schroeder, H., Müller, O., Niemeyer, C., Spengler, B., Goody, R., & Engelhard, M. (2005). Direct readout of protein-protein interactions by mass spectrometry from protein-DNA microarrays. Angewandte Chemie International Edition. 44(46), 7635-7639. https://doi.org/10.1002/anie.200502908


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