Journalartikel

Jahr der Veröffentlichung: 1993

Seiten: 91-98

Zeitschrift: Biotechnology Techniques

Bandnummer: 7

Heftnummer: 2

DOI Link: https://doi.org/10.1007/BF00157377

Verlag: Springer Verlag

Abstract: 

PIDDLE is a cloning strategy coupling antibody techniques to PCR.
Degenerate, multifunctional header-primers derived from peptide data
prime a single- or double-step PCR on genomic or cDNA template. Terminal
restriction sites are thereby created to allow efficient, in-frame,
directional cloning into the pBluescript-like pIDDLE phagemid. A
chimeric protein is then expressed in E.coli
and recognised by antibodies to the native protein. PIDDLE may also be
used to generate antibodies. We describe PIDDLE cloning of phy from oat.

Harvard-Zitierstil: Hughes, J., Qoronfleh, M., Bisztray, G. and Pratt, L. (1993) PIDDLE: PCR-based, in-frame, directional DNA ligation and expression, Biotechnology Techniques, 7(2), pp. 91-98. https://doi.org/10.1007/BF00157377

APA-Zitierstil: Hughes, J., Qoronfleh, M., Bisztray, G., & Pratt, L. (1993). PIDDLE: PCR-based, in-frame, directional DNA ligation and expression. Biotechnology Techniques. 7(2), 91-98. https://doi.org/10.1007/BF00157377