Journal article
Authors list: Hughes, JE; Qoronfleh, MW; Bisztray, G; Pratt, LH
Publication year: 1993
Pages: 91-98
Journal: Biotechnology Techniques
Volume number: 7
Issue number: 2
DOI Link: https://doi.org/10.1007/BF00157377
Publisher: Springer Verlag
PIDDLE is a cloning strategy coupling antibody techniques to PCR.
Abstract:
Degenerate, multifunctional header-primers derived from peptide data
prime a single- or double-step PCR on genomic or cDNA template. Terminal
restriction sites are thereby created to allow efficient, in-frame,
directional cloning into the pBluescript-like pIDDLE phagemid. A
chimeric protein is then expressed in E.coli
and recognised by antibodies to the native protein. PIDDLE may also be
used to generate antibodies. We describe PIDDLE cloning of phy from oat.
Citation Styles
Harvard Citation style: Hughes, J., Qoronfleh, M., Bisztray, G. and Pratt, L. (1993) PIDDLE: PCR-based, in-frame, directional DNA ligation and expression, Biotechnology Techniques, 7(2), pp. 91-98. https://doi.org/10.1007/BF00157377
APA Citation style: Hughes, J., Qoronfleh, M., Bisztray, G., & Pratt, L. (1993). PIDDLE: PCR-based, in-frame, directional DNA ligation and expression. Biotechnology Techniques. 7(2), 91-98. https://doi.org/10.1007/BF00157377
