Sammelbandbeitrag

Erschienen in: Virus-Induced Gene Silencing : Methods and Protocols

Herausgeberliste: Becker, A

Jahr der Veröffentlichung: 2013

Seiten: 1-14

ISBN: 978-1627032773

eISBN: 978-1-62703-278-0

DOI Link: https://doi.org/10.1007/978-1-62703-278-0_1

Serientitel: Methods in Molecular Biology

Serienzählung: 975

Abstract: 
The analysis of gene functions in non-model plant species is often
hampered by the fact that stable genetic transformation to downregulate
gene expression is laborious and time-consuming, or, for some species,
even not achievable. Virus-induced gene silencing (VIGS) can serve as an
alternative to mutant collections or stable transgenic plants to allow
the characterization of gene functions in a wide range of angiosperm
species, albeit in a transient way. VIGS vector systems have been
developed from both RNA and DNA plant viral sources to specifically
silence target genes in plants. VIGS is nowadays widely used in plant
genetics for gene knockdown due to its ease of use and the short time
required to generating phenotypes. Here, we summarize successfully
targeted eudicot and monocot plant species along with their specific
VIGS vector systems which are already available for researchers.

Harvard-Zitierstil: Lange, M., Yellina, A., Orashakova, S. and Becker, A. (2013) Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems, in Becker, A. (ed.) Virus-Induced Gene Silencing : Methods and Protocols. Totowa: Humana Press, pp. 1-14. https://doi.org/10.1007/978-1-62703-278-0_1

APA-Zitierstil: Lange, M., Yellina, A., Orashakova, S., & Becker, A. (2013). Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems. In Becker, A. (Ed.), Virus-Induced Gene Silencing : Methods and Protocols (pp. 1-14). Humana Press. https://doi.org/10.1007/978-1-62703-278-0_1