Contribution in an anthology

Appeared in: Virus-Induced Gene Silencing : Methods and Protocols

Editor list: Becker, A

Publication year: 2013

Pages: 1-14

ISBN: 978-1627032773

eISBN: 978-1-62703-278-0

DOI Link: https://doi.org/10.1007/978-1-62703-278-0_1

Title of series: Methods in Molecular Biology

Number in series: 975

Abstract: 
The analysis of gene functions in non-model plant species is often
hampered by the fact that stable genetic transformation to downregulate
gene expression is laborious and time-consuming, or, for some species,
even not achievable. Virus-induced gene silencing (VIGS) can serve as an
alternative to mutant collections or stable transgenic plants to allow
the characterization of gene functions in a wide range of angiosperm
species, albeit in a transient way. VIGS vector systems have been
developed from both RNA and DNA plant viral sources to specifically
silence target genes in plants. VIGS is nowadays widely used in plant
genetics for gene knockdown due to its ease of use and the short time
required to generating phenotypes. Here, we summarize successfully
targeted eudicot and monocot plant species along with their specific
VIGS vector systems which are already available for researchers.

Harvard Citation style: Lange, M., Yellina, A., Orashakova, S. and Becker, A. (2013) Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems, in Becker, A. (ed.) Virus-Induced Gene Silencing : Methods and Protocols. Totowa: Humana Press, pp. 1-14. https://doi.org/10.1007/978-1-62703-278-0_1

APA Citation style: Lange, M., Yellina, A., Orashakova, S., & Becker, A. (2013). Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems. In Becker, A. (Ed.), Virus-Induced Gene Silencing : Methods and Protocols (pp. 1-14). Humana Press. https://doi.org/10.1007/978-1-62703-278-0_1