Journalartikel

Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide


AutorenlisteBosisio, D; Polentarutti, N; Sironi, M; Bernasconi, S; Miyake, K; Webb, GR; Martin, MU; Mantovani, A; Muzio, M

Jahr der Veröffentlichung2002

Seiten3427-3431

ZeitschriftBlood

Bandnummer99

Heftnummer9

ISSN0006-4971

DOI Linkhttps://doi.org/10.1182/blood.V99.9.3427

VerlagAmerican Society of Hematology (ASH Publications)


Abstract
In human monocytes and macrophages, Interferon-gamma (IFNgamma) augmented mRNA and surface expression of toll-like receptor 4 (TLR4), a crucial component of the signaling receptor complex for bacterial lipopolysaccharide (LPS). Expression of the accessory component MD-2 and of the adapter protein MyD88 was also increased. LPS increased TLR4 mRNA levels, but concomitantly decreased its surface expression. IFNgamma counteracted the LPS-induced downregulation of TLR4. IFNgamma-primed monocytes showed increased responsiveness to LPS in terms of phosphorylation of the interieukin-1 receptor-associated kinase (IRAK; immediately downstream of the MyD88 adapter protein), NF-kB DNA binding activity, and, accordingly, of cytokine (tumor necrosis factor alpha [TNFalpha] and interieukin-12 [IL-12]) production. These results suggest that enhanced TLR4 expression underlies the long-known priming by IFNgamma of mononuclear phagocytes for pathogen recognition and killing as well as its synergism with LPS in macrophage activation. (C) 2002 by The American Society of Hematology.



Zitierstile

Harvard-ZitierstilBosisio, D., Polentarutti, N., Sironi, M., Bernasconi, S., Miyake, K., Webb, G., et al. (2002) Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide, Blood, 99(9), pp. 3427-3431. https://doi.org/10.1182/blood.V99.9.3427

APA-ZitierstilBosisio, D., Polentarutti, N., Sironi, M., Bernasconi, S., Miyake, K., Webb, G., Martin, M., Mantovani, A., & Muzio, M. (2002). Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide. Blood. 99(9), 3427-3431. https://doi.org/10.1182/blood.V99.9.3427


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