Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide - Research portal of Justus Liebig University Giessen:

Journal article

Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide

Authors list: Bosisio, D; Polentarutti, N; Sironi, M; Bernasconi, S; Miyake, K; Webb, GR; Martin, MU; Mantovani, A; Muzio, M

Publication year: 2002

Pages: 3427-3431

Journal: Blood

Volume number: 99

Issue number: 9

ISSN: 0006-4971

DOI Link: https://doi.org/10.1182/blood.V99.9.3427

Publisher: American Society of Hematology (ASH Publications)

Abstract:
In human monocytes and macrophages, Interferon-gamma (IFNgamma) augmented mRNA and surface expression of toll-like receptor 4 (TLR4), a crucial component of the signaling receptor complex for bacterial lipopolysaccharide (LPS). Expression of the accessory component MD-2 and of the adapter protein MyD88 was also increased. LPS increased TLR4 mRNA levels, but concomitantly decreased its surface expression. IFNgamma counteracted the LPS-induced downregulation of TLR4. IFNgamma-primed monocytes showed increased responsiveness to LPS in terms of phosphorylation of the interieukin-1 receptor-associated kinase (IRAK; immediately downstream of the MyD88 adapter protein), NF-kB DNA binding activity, and, accordingly, of cytokine (tumor necrosis factor alpha [TNFalpha] and interieukin-12 [IL-12]) production. These results suggest that enhanced TLR4 expression underlies the long-known priming by IFNgamma of mononuclear phagocytes for pathogen recognition and killing as well as its synergism with LPS in macrophage activation. (C) 2002 by The American Society of Hematology.

Citation Styles

Harvard Citation style: Bosisio, D., Polentarutti, N., Sironi, M., Bernasconi, S., Miyake, K., Webb, G., et al. (2002) Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide, Blood, 99(9), pp. 3427-3431. https://doi.org/10.1182/blood.V99.9.3427

APA Citation style: Bosisio, D., Polentarutti, N., Sironi, M., Bernasconi, S., Miyake, K., Webb, G., Martin, M., Mantovani, A., & Muzio, M. (2002). Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-γ: a molecular basis for priming and synergism with bacterial lipopolysaccharide. Blood. 99(9), 3427-3431. https://doi.org/10.1182/blood.V99.9.3427