Journalartikel

Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya


AutorenlisteClewing, C; Schultheiß, R; Wilke, T; Albrecht, C

Jahr der Veröffentlichung2013

Seiten51-54

ZeitschriftConservation Genetics Resources

Bandnummer5

Heftnummer1

ISSN1877-7252

DOI Linkhttps://doi.org/10.1007/s12686-012-9731-5

VerlagSpringer


Abstract
We isolated and characterized microsatellite markers for African species of the freshwater gastropod genus Bellamya. Fifteen out of 21 primer combinations, isolated from unpublished sequences, yielded in polymorphic products. The number of alleles ranged from 8 to 22 among the 37 scored individuals. The observed heterozygosity H (O) ranged from 0.571 to 0.919 and the expected heterozygosity H (E) ranged from 0.626 to 0.937. Evidence of null alleles were observed at three polymorphic loci. Cross-species amplifications were carried out for five additional African viviparid species and resulted in several polymorphic microsatellite markers, a high number of monomorphic loci as well as a few unsuccessful amplifications.



Autoren/Herausgeber




Zitierstile

Harvard-ZitierstilClewing, C., Schultheiß, R., Wilke, T. and Albrecht, C. (2013) Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya, Conservation Genetics Resources, 5(1), pp. 51-54. https://doi.org/10.1007/s12686-012-9731-5

APA-ZitierstilClewing, C., Schultheiß, R., Wilke, T., & Albrecht, C. (2013). Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya. Conservation Genetics Resources. 5(1), 51-54. https://doi.org/10.1007/s12686-012-9731-5



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