Journal article

Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya


Authors listClewing, C; Schultheiß, R; Wilke, T; Albrecht, C

Publication year2013

Pages51-54

JournalConservation Genetics Resources

Volume number5

Issue number1

ISSN1877-7252

DOI Linkhttps://doi.org/10.1007/s12686-012-9731-5

PublisherSpringer


Abstract
We isolated and characterized microsatellite markers for African species of the freshwater gastropod genus Bellamya. Fifteen out of 21 primer combinations, isolated from unpublished sequences, yielded in polymorphic products. The number of alleles ranged from 8 to 22 among the 37 scored individuals. The observed heterozygosity H (O) ranged from 0.571 to 0.919 and the expected heterozygosity H (E) ranged from 0.626 to 0.937. Evidence of null alleles were observed at three polymorphic loci. Cross-species amplifications were carried out for five additional African viviparid species and resulted in several polymorphic microsatellite markers, a high number of monomorphic loci as well as a few unsuccessful amplifications.



Authors/Editors




Citation Styles

Harvard Citation styleClewing, C., Schultheiß, R., Wilke, T. and Albrecht, C. (2013) Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya, Conservation Genetics Resources, 5(1), pp. 51-54. https://doi.org/10.1007/s12686-012-9731-5

APA Citation styleClewing, C., Schultheiß, R., Wilke, T., & Albrecht, C. (2013). Isolation, characterization and cross-species amplification of 15 microsatellite markers for the African species of the freshwater gastropod genus Bellamya. Conservation Genetics Resources. 5(1), 51-54. https://doi.org/10.1007/s12686-012-9731-5



SDG Areas


Last updated on 2025-21-05 at 15:23