Journalartikel

Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area


AutorenlisteRowe, C; Ittiprasert, W; Patterson, C; Eliff, C; Page, K; Bandoni, S; Wilke, T; Minchella, D; Lewis, F; Knight, M

Jahr der Veröffentlichung2003

Seiten149-166

ZeitschriftMalacologia

Bandnummer45

Heftnummer1

ISSN0076-2997

URLhttps://www.biodiversitylibrary.org/item/81074#page/157/mode/1up

VerlagInstitute of Malacology


Abstract
Genetic variation was assessed in Biomphalaria glabrata snails using variations in microsatellite loci and by RAPD-PCR analysis. Populations of snails examined were field-collected isolates from a small pond in a schistosomiasis endemic region in Brazil, after standard conditions were developed for analyzing snails from two laboratory-maintained stocks. The analyses were performed using a total of 60 microsatellite primer sets and, for RAPD-PCR, a total of 19 random primers. We show that genetic diversity can readily be detected by both molecular methods among the field-collected snails from this small site. In addition, RAPD-PCR bands that were found in another study to segregate with parasite resistance were not detected in any of the field-collected snails analyzed.



Autoren/Herausgeber




Zitierstile

Harvard-ZitierstilRowe, C., Ittiprasert, W., Patterson, C., Eliff, C., Page, K., Bandoni, S., et al. (2003) Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area, Malacologia, 45(1), pp. 149-166. https://www.biodiversitylibrary.org/item/81074#page/157/mode/1up

APA-ZitierstilRowe, C., Ittiprasert, W., Patterson, C., Eliff, C., Page, K., Bandoni, S., Wilke, T., Minchella, D., Lewis, F., & Knight, M. (2003). Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area. Malacologia. 45(1), 149-166. https://www.biodiversitylibrary.org/item/81074#page/157/mode/1up


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