Journal article

Publication year: 2003

Pages: 149-166

Journal: Malacologia

Volume number: 45

Issue number: 1

ISSN: 0076-2997

URL: https://www.biodiversitylibrary.org/item/81074#page/157/mode/1up

Publisher: Institute of Malacology

Abstract: 
Genetic variation was assessed in Biomphalaria glabrata snails using variations in microsatellite loci and by RAPD-PCR analysis. Populations of snails examined were field-collected isolates from a small pond in a schistosomiasis endemic region in Brazil, after standard conditions were developed for analyzing snails from two laboratory-maintained stocks. The analyses were performed using a total of 60 microsatellite primer sets and, for RAPD-PCR, a total of 19 random primers. We show that genetic diversity can readily be detected by both molecular methods among the field-collected snails from this small site. In addition, RAPD-PCR bands that were found in another study to segregate with parasite resistance were not detected in any of the field-collected snails analyzed.

Harvard Citation style: Rowe, C., Ittiprasert, W., Patterson, C., Eliff, C., Page, K., Bandoni, S., et al. (2003) Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area, Malacologia, 45(1), pp. 149-166. https://www.biodiversitylibrary.org/item/81074#page/157/mode/1up

APA Citation style: Rowe, C., Ittiprasert, W., Patterson, C., Eliff, C., Page, K., Bandoni, S., Wilke, T., Minchella, D., Lewis, F., & Knight, M. (2003). Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area. Malacologia. 45(1), 149-166. https://www.biodiversitylibrary.org/item/81074#page/157/mode/1up