Journalartikel

Jahr der Veröffentlichung: 1996

Seiten: 831-838

Zeitschrift: Biotechnology Techniques

Bandnummer: 10

Heftnummer: 11

ISSN: 0951-208X

DOI Link: https://doi.org/10.1007/BF00154667

Verlag: Springer Verlag

Abstract: 
The pIDDLE6 (for ECR-based, in-frame, directional DNA ligation and expression) system utilises novel ligation procedures to clone and overexpress almost any coding sequence. Inserts are cloned either by blunt ligation in the presence of PmeI or by ligation between dissimilar SfiI sites. A chimeric protein with both N- and C-terminal tags is produced, allowing purification by via starch-or nickel-affinity under native or denaturing conditions. The tags can be removed independently. The the vector and ligation procedures have been used successfully as described.

Harvard-Zitierstil: Hughes, J. (1996) pIDDLE6: A system for ligation and expression-cloning in E.coli, Biotechnology Techniques, 10(11), pp. 831-838. https://doi.org/10.1007/BF00154667

APA-Zitierstil: Hughes, J. (1996). pIDDLE6: A system for ligation and expression-cloning in E.coli. Biotechnology Techniques. 10(11), 831-838. https://doi.org/10.1007/BF00154667