Journal article

Publication year: 1996

Pages: 831-838

Journal: Biotechnology Techniques

Volume number: 10

Issue number: 11

ISSN: 0951-208X

DOI Link: https://doi.org/10.1007/BF00154667

Publisher: Springer Verlag

Abstract: 
The pIDDLE6 (for ECR-based, in-frame, directional DNA ligation and expression) system utilises novel ligation procedures to clone and overexpress almost any coding sequence. Inserts are cloned either by blunt ligation in the presence of PmeI or by ligation between dissimilar SfiI sites. A chimeric protein with both N- and C-terminal tags is produced, allowing purification by via starch-or nickel-affinity under native or denaturing conditions. The tags can be removed independently. The the vector and ligation procedures have been used successfully as described.

Harvard Citation style: Hughes, J. (1996) pIDDLE6: A system for ligation and expression-cloning in E.coli, Biotechnology Techniques, 10(11), pp. 831-838. https://doi.org/10.1007/BF00154667

APA Citation style: Hughes, J. (1996). pIDDLE6: A system for ligation and expression-cloning in E.coli. Biotechnology Techniques. 10(11), 831-838. https://doi.org/10.1007/BF00154667