Journalartikel

Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei


AutorenlistePreußer, C; Palfi, Z; Bindereif, A

Jahr der Veröffentlichung2009

Seiten1228-1234

ZeitschriftEukaryotic Cell

Bandnummer8

Heftnummer8

ISSN1535-9778

eISSN1535-9786

Open Access StatusGold

DOI Linkhttps://doi.org/10.1128/EC.00090-09

VerlagAmerican Society for Microbiology


Abstract
The processing of polycistronic pre-mRNAs in trypanosomes requires the spliceosomal small ribonucleoprotein complexes (snRNPs) U1, U2, U4/U6, U5, and SL, each of which contains a core of seven Sm proteins. Recently we reported the first evidence for a core variation in spliceosomal snRNPs; specifically, in the trypanosome U2 snRNP, two of the canonical Sm proteins, SmB and SmD3, are replaced by two U2-specific Sm proteins, Sm15K and Sm16.5K. Here we identify the U2-specific, nuclear-localized U2B '' protein from Trypanosoma brucei. U2B '' interacts with a second U2 snRNP protein, U2-40K (U2A'), which in turn contacts the U2-specific Sm16.5K/15K subcomplex. Together they form a high-affinity, U2-specific binding complex. This trypanosome-specific assembly differs from the mammalian system and provides a functional role for the Sm core variation found in the trypanosomal U2 snRNP.



Zitierstile

Harvard-ZitierstilPreußer, C., Palfi, Z. and Bindereif, A. (2009) Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei, Eukaryotic Cell, 8(8), pp. 1228-1234. https://doi.org/10.1128/EC.00090-09

APA-ZitierstilPreußer, C., Palfi, Z., & Bindereif, A. (2009). Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei. Eukaryotic Cell. 8(8), 1228-1234. https://doi.org/10.1128/EC.00090-09



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