Journal article

Publication year: 2009

Pages: 1228-1234

Journal: Eukaryotic Cell

Volume number: 8

Issue number: 8

ISSN: 1535-9778

eISSN: 1535-9786

Open access status: Gold

DOI Link: https://doi.org/10.1128/EC.00090-09

Publisher: American Society for Microbiology

Abstract: 
The processing of polycistronic pre-mRNAs in trypanosomes requires the spliceosomal small ribonucleoprotein complexes (snRNPs) U1, U2, U4/U6, U5, and SL, each of which contains a core of seven Sm proteins. Recently we reported the first evidence for a core variation in spliceosomal snRNPs; specifically, in the trypanosome U2 snRNP, two of the canonical Sm proteins, SmB and SmD3, are replaced by two U2-specific Sm proteins, Sm15K and Sm16.5K. Here we identify the U2-specific, nuclear-localized U2B '' protein from Trypanosoma brucei. U2B '' interacts with a second U2 snRNP protein, U2-40K (U2A'), which in turn contacts the U2-specific Sm16.5K/15K subcomplex. Together they form a high-affinity, U2-specific binding complex. This trypanosome-specific assembly differs from the mammalian system and provides a functional role for the Sm core variation found in the trypanosomal U2 snRNP.

Harvard Citation style: Preußer, C., Palfi, Z. and Bindereif, A. (2009) Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei, Eukaryotic Cell, 8(8), pp. 1228-1234. https://doi.org/10.1128/EC.00090-09

APA Citation style: Preußer, C., Palfi, Z., & Bindereif, A. (2009). Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei. Eukaryotic Cell. 8(8), 1228-1234. https://doi.org/10.1128/EC.00090-09