Journalartikel

Jahr der Veröffentlichung: 1990

Seiten: 251-255

Zeitschrift: The EMBO Journal

Bandnummer: 9

Heftnummer: 1

ISSN: 0261-4189

eISSN: 1460-2075

URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/

Verlag: EMBO Press

Abstract: 
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

Harvard-Zitierstil: Bindereif, A., Wolff, T. and Green, M. (1990) Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes., The EMBO Journal, 9(1), pp. 251-255. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/

APA-Zitierstil: Bindereif, A., Wolff, T., & Green, M. (1990). Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.. The EMBO Journal. 9(1), 251-255. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/