Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes. - Research portal of Justus Liebig University Giessen:

Journal article

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.

Authors list: Bindereif, A; Wolff, T; Green, MR

Publication year: 1990

Pages: 251-255

Journal: The EMBO Journal

Volume number: 9

Issue number: 1

ISSN: 0261-4189

eISSN: 1460-2075

URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/

Publisher: EMBO Press

Abstract:
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

Citation Styles

Harvard Citation style: Bindereif, A., Wolff, T. and Green, M. (1990) Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes., The EMBO Journal, 9(1), pp. 251-255. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/

APA Citation style: Bindereif, A., Wolff, T., & Green, M. (1990). Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.. The EMBO Journal. 9(1), 251-255. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551655/