Journalartikel
Autorenliste: Heckenhauer, Jacqueline; Razuri-Gonzales, Ernesto; Mwangi, Francois Ngera; Schneider, Julio; Pauls, Steffen U.
Jahr der Veröffentlichung: 2023
Seiten: 1-15
Zeitschrift: ZooKeys
Heftnummer: 1159
ISSN: 1313-2989
eISSN: 1313-2970
Open Access Status: Gold
DOI Link: https://doi.org/10.3897/zookeys.1159.98439
Verlag: Pensoft Publishers
Abstract:
While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies.
Zitierstile
Harvard-Zitierstil: Heckenhauer, J., Razuri-Gonzales, E., Mwangi, F., Schneider, J. and Pauls, S. (2023) Holotype sequencing of Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae), ZooKeys(1159), pp. 1-15. https://doi.org/10.3897/zookeys.1159.98439
APA-Zitierstil: Heckenhauer, J., Razuri-Gonzales, E., Mwangi, F., Schneider, J., & Pauls, S. (2023). Holotype sequencing of Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae). ZooKeys(1159), 1-15. https://doi.org/10.3897/zookeys.1159.98439
Schlagwörter
Caddisflies; extended specimen; holotype genomics; INSECTA