Journal article

Publication year: 2023

Pages: 1-15

Journal: ZooKeys

Issue number: 1159

ISSN: 1313-2989

eISSN: 1313-2970

Open access status: Gold

DOI Link: https://doi.org/10.3897/zookeys.1159.98439

Publisher: Pensoft Publishers

Abstract: 
While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies.

Harvard Citation style: Heckenhauer, J., Razuri-Gonzales, E., Mwangi, F., Schneider, J. and Pauls, S. (2023) Holotype sequencing of Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae), ZooKeys(1159), pp. 1-15. https://doi.org/10.3897/zookeys.1159.98439

APA Citation style: Heckenhauer, J., Razuri-Gonzales, E., Mwangi, F., Schneider, J., & Pauls, S. (2023). Holotype sequencing of Silvatares holzenthali Razuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae). ZooKeys(1159), 1-15. https://doi.org/10.3897/zookeys.1159.98439