Journalartikel

The film-forming Pichia spp. in a winemaker's toolbox: A simple isolation procedure and their performance in a mixed-culture fermentation of Vitis vinifera L. cv. Gewurztraminer must


AutorenlisteScansani, Stefano; van Wyk, Niel; Nader, Khalil Bou; Beisert, Beata; Brezina, Silvia; Fritsch, Stefanie; Semmler, Heike; Pasch, Ludwig; Pretorius, Isak S.; von Wallbrunn, Christian; Schnell, Sylvia; Rauhut, Doris

Jahr der Veröffentlichung2022

ZeitschriftInternational Journal of Food Microbiology

Bandnummer365

ISSN0168-1605

eISSN1879-3460

DOI Linkhttps://doi.org/10.1016/j.ijfoodmicro.2022.109549

VerlagElsevier


Abstract
Certain yeast species belonging to the Pichia genus are known to form a distinctive film on grape must and wine. In a mixed-culture type fermentation, Pichia spp. (P. kluyveri in particular) are known to impart beneficial oenological attributes. In this study, we report on an easy isolation method of Pichia spp. from grape must by exploiting their film-forming capacity on media containing 10% ethanol. We isolated and identified two Pichia species, namely Pichia kudriavzevii and Pichia kluyveri, and subsequently co-inoculated them with Saccharomyces cerevisiae to ferment Gewurztraminer musts. Noteworthy differences included a significant increase in the 2-phe-nethyl acetate levels with the P. kluyveri co-fermentation and a general increase in ethyl esters with the P. kudriavzevii co-fermentation. Both Pichia co-inoculations yielded higher levels of glycerol in the final wines. Based on all the wine parameters we tested, the P. kluyveri strain that was isolated performed similarly to a commercial P. kluyveri strain.



Zitierstile

Harvard-ZitierstilScansani, S., van Wyk, N., Nader, K., Beisert, B., Brezina, S., Fritsch, S., et al. (2022) The film-forming Pichia spp. in a winemaker's toolbox: A simple isolation procedure and their performance in a mixed-culture fermentation of Vitis vinifera L. cv. Gewurztraminer must, International Journal of Food Microbiology, 365, Article 109549. https://doi.org/10.1016/j.ijfoodmicro.2022.109549

APA-ZitierstilScansani, S., van Wyk, N., Nader, K., Beisert, B., Brezina, S., Fritsch, S., Semmler, H., Pasch, L., Pretorius, I., von Wallbrunn, C., Schnell, S., & Rauhut, D. (2022). The film-forming Pichia spp. in a winemaker's toolbox: A simple isolation procedure and their performance in a mixed-culture fermentation of Vitis vinifera L. cv. Gewurztraminer must. International Journal of Food Microbiology. 365, Article 109549. https://doi.org/10.1016/j.ijfoodmicro.2022.109549



Schlagwörter


CEREVISIAECO-FERMENTATIONFILM FORMATIONGewurztraminerGlycerolGRAPEISSATCHENKIA-ORIENTALISMALIC-ACIDNonNON-SACCHAROMYCES YEASTSPhenethyl acetatePichia kluyveriPichia kudriavzeviiRED WINER-PACKAGESaccharomyces yeastsspoilageWine fermentation


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