Journal article
Authors list: Scansani, Stefano; van Wyk, Niel; Nader, Khalil Bou; Beisert, Beata; Brezina, Silvia; Fritsch, Stefanie; Semmler, Heike; Pasch, Ludwig; Pretorius, Isak S.; von Wallbrunn, Christian; Schnell, Sylvia; Rauhut, Doris
Publication year: 2022
Journal: International Journal of Food Microbiology
Volume number: 365
ISSN: 0168-1605
eISSN: 1879-3460
DOI Link: https://doi.org/10.1016/j.ijfoodmicro.2022.109549
Publisher: Elsevier
Abstract:
Certain yeast species belonging to the Pichia genus are known to form a distinctive film on grape must and wine. In a mixed-culture type fermentation, Pichia spp. (P. kluyveri in particular) are known to impart beneficial oenological attributes. In this study, we report on an easy isolation method of Pichia spp. from grape must by exploiting their film-forming capacity on media containing 10% ethanol. We isolated and identified two Pichia species, namely Pichia kudriavzevii and Pichia kluyveri, and subsequently co-inoculated them with Saccharomyces cerevisiae to ferment Gewurztraminer musts. Noteworthy differences included a significant increase in the 2-phe-nethyl acetate levels with the P. kluyveri co-fermentation and a general increase in ethyl esters with the P. kudriavzevii co-fermentation. Both Pichia co-inoculations yielded higher levels of glycerol in the final wines. Based on all the wine parameters we tested, the P. kluyveri strain that was isolated performed similarly to a commercial P. kluyveri strain.
Citation Styles
Harvard Citation style: Scansani, S., van Wyk, N., Nader, K., Beisert, B., Brezina, S., Fritsch, S., et al. (2022) The film-forming Pichia spp. in a winemaker's toolbox: A simple isolation procedure and their performance in a mixed-culture fermentation of Vitis vinifera L. cv. Gewurztraminer must, International Journal of Food Microbiology, 365, Article 109549. https://doi.org/10.1016/j.ijfoodmicro.2022.109549
APA Citation style: Scansani, S., van Wyk, N., Nader, K., Beisert, B., Brezina, S., Fritsch, S., Semmler, H., Pasch, L., Pretorius, I., von Wallbrunn, C., Schnell, S., & Rauhut, D. (2022). The film-forming Pichia spp. in a winemaker's toolbox: A simple isolation procedure and their performance in a mixed-culture fermentation of Vitis vinifera L. cv. Gewurztraminer must. International Journal of Food Microbiology. 365, Article 109549. https://doi.org/10.1016/j.ijfoodmicro.2022.109549
Keywords
CEREVISIAE; CO-FERMENTATION; FILM FORMATION; Gewurztraminer; Glycerol; GRAPE; ISSATCHENKIA-ORIENTALIS; MALIC-ACID; Non; NON-SACCHAROMYCES YEASTS; Phenethyl acetate; Pichia kluyveri; Pichia kudriavzevii; RED WINE; R-PACKAGE; Saccharomyces yeasts; spoilage; Wine fermentation
