Journalartikel

Jahr der Veröffentlichung: 2020

Zeitschrift: International Journal of Antimicrobial Agents

Bandnummer: 56

Heftnummer: 6

ISSN: 0924-8579

eISSN: 1872-7913

Open Access Status: Green

DOI Link: https://doi.org/10.1016/j.ijantimicag.2020.106157

Verlag: Elsevier

Abstract: 

Background: Carbapenemase-producing Gram-negative bacteria cause infections that are difficult to treat and represent a rising threat to healthcare systems worldwide. This study analysed isolates of Escherichia coli (E. coli), a species associated with nosocomial-acquired and community-acquired infections, from hospitals in Germany and Switzerland exhibiting a slight decrease in susceptibility to carbapenems.

Methods: E. coli strains from Germany and Switzerland, obtained mainly in 2019, were first screened for carbapenemase genes by PCR and subsequently whole-genome-sequenced and analysed for their clonal relationship using multilocus sequence typing, single nucleotide polymorphisms, virulence and antibioticresistance gene content.

Results: The analysis revealed the presence of extended beta-lactamase (ESBL)-producing E. coli clones producing OXA-244, a point-mutation derivative of OXA-48, with a predominance of isolates exhibiting the sequence type (ST) ST38 in both Germany and Switzerland. These clustered exclusively into two distinct lineages: one encoding CTX-M-27, a recently emerged extended-spectrum beta-lactamase, and the other CTX-M-14b. All OXA244/CTX-M-27 ST38 isolates harboured the Dr adhesin operon and a representative isolate exhibited a diffuse adherence (DAEC) phenotype and was invasive for Hela cells.

Conclusion: Clonal lineages of ST38 are members of E. coli phylogenetic group D commonly associated with extra-intestinal infections. Their increased isolation in two different European countries indicates ongoing spread of ST38 ESBL-producing and OXA-244-producing E. coli clonal lineages. It is possible that members of the multidrug-resistant DEAC ExPEC group have expanded globally, but that this is currently underreported because of the inherent difficulty in detecting isolates expressing the OXA-244 allele. (C) 2020 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.

Harvard-Zitierstil: Falgenhauer, L., Nordmann, P., Imirzalioglu, C., Yao, Y., Falgenhauer, J., Hauri, A., et al. (2020) Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland, International Journal of Antimicrobial Agents, 56(6), Article 106157. https://doi.org/10.1016/j.ijantimicag.2020.106157

APA-Zitierstil: Falgenhauer, L., Nordmann, P., Imirzalioglu, C., Yao, Y., Falgenhauer, J., Hauri, A., Heinmueller, P., & Chakraborty, T. (2020). Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland. International Journal of Antimicrobial Agents. 56(6), Article 106157. https://doi.org/10.1016/j.ijantimicag.2020.106157