Journal article

Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland


Authors listFalgenhauer, Linda; Nordmann, Patrice; Imirzalioglu, Can; Yao, Yancheng; Falgenhauer, Jane; Hauri, Anja M.; Heinmueller, Petra; Chakraborty, Trinad

Publication year2020

JournalInternational Journal of Antimicrobial Agents

Volume number56

Issue number6

ISSN0924-8579

eISSN1872-7913

Open access statusGreen

DOI Linkhttps://doi.org/10.1016/j.ijantimicag.2020.106157

PublisherElsevier


Abstract

Background: Carbapenemase-producing Gram-negative bacteria cause infections that are difficult to treat and represent a rising threat to healthcare systems worldwide. This study analysed isolates of Escherichia coli (E. coli), a species associated with nosocomial-acquired and community-acquired infections, from hospitals in Germany and Switzerland exhibiting a slight decrease in susceptibility to carbapenems.

Methods: E. coli strains from Germany and Switzerland, obtained mainly in 2019, were first screened for carbapenemase genes by PCR and subsequently whole-genome-sequenced and analysed for their clonal relationship using multilocus sequence typing, single nucleotide polymorphisms, virulence and antibioticresistance gene content.

Results: The analysis revealed the presence of extended beta-lactamase (ESBL)-producing E. coli clones producing OXA-244, a point-mutation derivative of OXA-48, with a predominance of isolates exhibiting the sequence type (ST) ST38 in both Germany and Switzerland. These clustered exclusively into two distinct lineages: one encoding CTX-M-27, a recently emerged extended-spectrum beta-lactamase, and the other CTX-M-14b. All OXA244/CTX-M-27 ST38 isolates harboured the Dr adhesin operon and a representative isolate exhibited a diffuse adherence (DAEC) phenotype and was invasive for Hela cells.

Conclusion: Clonal lineages of ST38 are members of E. coli phylogenetic group D commonly associated with extra-intestinal infections. Their increased isolation in two different European countries indicates ongoing spread of ST38 ESBL-producing and OXA-244-producing E. coli clonal lineages. It is possible that members of the multidrug-resistant DEAC ExPEC group have expanded globally, but that this is currently underreported because of the inherent difficulty in detecting isolates expressing the OXA-244 allele. (C) 2020 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.




Citation Styles

Harvard Citation styleFalgenhauer, L., Nordmann, P., Imirzalioglu, C., Yao, Y., Falgenhauer, J., Hauri, A., et al. (2020) Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland, International Journal of Antimicrobial Agents, 56(6), Article 106157. https://doi.org/10.1016/j.ijantimicag.2020.106157

APA Citation styleFalgenhauer, L., Nordmann, P., Imirzalioglu, C., Yao, Y., Falgenhauer, J., Hauri, A., Heinmueller, P., & Chakraborty, T. (2020). Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland. International Journal of Antimicrobial Agents. 56(6), Article 106157. https://doi.org/10.1016/j.ijantimicag.2020.106157



Keywords


Carbapenemase-producersCTX-M-27/14bDiffusely adhering E. coli (DAEC)OXA-244

Last updated on 2025-10-06 at 11:19