Journalartikel

Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants


AutorenlisteNausch, Henrik; Huckauf, Jana; Broer, Inge

Jahr der Veröffentlichung2016

Seiten1559-1565

ZeitschriftApplied Microbiology and Biotechnology

Bandnummer100

Heftnummer4

ISSN0175-7598

eISSN1432-0614

DOI Linkhttps://doi.org/10.1007/s00253-015-7212-y

VerlagSpringer


Abstract
Cyanophycin (CP) can be successfully produced in plants by the ectopic expression of the CphA synthetase from Thermosynechococcus elongatus BP-1 (Berg et al. 2000), yielding up to 6.8 % of dry weight (DW) in tobacco leaf tissue and 7.5 % in potato tubers (Huehns et al. 2008, 2009). Though, high amounts of the polymer lead to phenotypical abnormalities in both crops. The extension of abnormalities and the maximum amount of CP tolerated depend on the compartment that CP production is localized at the tissue/crop in which CP was produced (Huehns et al. 2008, 2009; Neumann et al. 2005). It cannot be ascribed to a depletion of arginine, lysine, or aspartate, the substrates for CP synthesis.



Zitierstile

Harvard-ZitierstilNausch, H., Huckauf, J. and Broer, I. (2016) Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants, Applied Microbiology and Biotechnology, 100(4), pp. 1559-1565. https://doi.org/10.1007/s00253-015-7212-y

APA-ZitierstilNausch, H., Huckauf, J., & Broer, I. (2016). Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants. Applied Microbiology and Biotechnology. 100(4), 1559-1565. https://doi.org/10.1007/s00253-015-7212-y



Schlagwörter


ASPARTIC-ACIDCphADIPEPTIDESGRANULE POLYPEPTIDENicotiana tabacumPlastidsPOLYHYDROXYBUTYRATERALSTONIA-EUTROPHARECOMBINANT STRAINSSolanum tuberosumThermosynechococcus elongates BP-1

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