Journal article

Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants


Authors listNausch, Henrik; Huckauf, Jana; Broer, Inge

Publication year2016

Pages1559-1565

JournalApplied Microbiology and Biotechnology

Volume number100

Issue number4

ISSN0175-7598

eISSN1432-0614

DOI Linkhttps://doi.org/10.1007/s00253-015-7212-y

PublisherSpringer


Abstract
Cyanophycin (CP) can be successfully produced in plants by the ectopic expression of the CphA synthetase from Thermosynechococcus elongatus BP-1 (Berg et al. 2000), yielding up to 6.8 % of dry weight (DW) in tobacco leaf tissue and 7.5 % in potato tubers (Huehns et al. 2008, 2009). Though, high amounts of the polymer lead to phenotypical abnormalities in both crops. The extension of abnormalities and the maximum amount of CP tolerated depend on the compartment that CP production is localized at the tissue/crop in which CP was produced (Huehns et al. 2008, 2009; Neumann et al. 2005). It cannot be ascribed to a depletion of arginine, lysine, or aspartate, the substrates for CP synthesis.



Citation Styles

Harvard Citation styleNausch, H., Huckauf, J. and Broer, I. (2016) Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants, Applied Microbiology and Biotechnology, 100(4), pp. 1559-1565. https://doi.org/10.1007/s00253-015-7212-y

APA Citation styleNausch, H., Huckauf, J., & Broer, I. (2016). Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants. Applied Microbiology and Biotechnology. 100(4), 1559-1565. https://doi.org/10.1007/s00253-015-7212-y



Keywords


ASPARTIC-ACIDCphADIPEPTIDESGRANULE POLYPEPTIDENicotiana tabacumPlastidsPOLYHYDROXYBUTYRATERALSTONIA-EUTROPHARECOMBINANT STRAINSSolanum tuberosumThermosynechococcus elongates BP-1

Last updated on 2025-02-04 at 01:53