Journal article
Authors list: Nausch, Henrik; Huckauf, Jana; Broer, Inge
Publication year: 2016
Pages: 1559-1565
Journal: Applied Microbiology and Biotechnology
Volume number: 100
Issue number: 4
ISSN: 0175-7598
eISSN: 1432-0614
DOI Link: https://doi.org/10.1007/s00253-015-7212-y
Publisher: Springer
Abstract:
Cyanophycin (CP) can be successfully produced in plants by the ectopic expression of the CphA synthetase from Thermosynechococcus elongatus BP-1 (Berg et al. 2000), yielding up to 6.8 % of dry weight (DW) in tobacco leaf tissue and 7.5 % in potato tubers (Huehns et al. 2008, 2009). Though, high amounts of the polymer lead to phenotypical abnormalities in both crops. The extension of abnormalities and the maximum amount of CP tolerated depend on the compartment that CP production is localized at the tissue/crop in which CP was produced (Huehns et al. 2008, 2009; Neumann et al. 2005). It cannot be ascribed to a depletion of arginine, lysine, or aspartate, the substrates for CP synthesis.
Citation Styles
Harvard Citation style: Nausch, H., Huckauf, J. and Broer, I. (2016) Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants, Applied Microbiology and Biotechnology, 100(4), pp. 1559-1565. https://doi.org/10.1007/s00253-015-7212-y
APA Citation style: Nausch, H., Huckauf, J., & Broer, I. (2016). Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants. Applied Microbiology and Biotechnology. 100(4), 1559-1565. https://doi.org/10.1007/s00253-015-7212-y
Keywords
ASPARTIC-ACID; CphA; DIPEPTIDES; GRANULE POLYPEPTIDE; Nicotiana tabacum; Plastids; POLYHYDROXYBUTYRATE; RALSTONIA-EUTROPHA; RECOMBINANT STRAINS; Solanum tuberosum; Thermosynechococcus elongates BP-1