Journalartikel

The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline


AutorenlisteHundt, Matthias; Schreiber, Benjamin; Eckmann, Reiner; Lunestad, Bjorn Tore; Wuenneman, Hannah; Schulz, Ralf

Jahr der Veröffentlichung2016

Seiten144-149

ZeitschriftBulletin of Environmental Contamination and Toxicology

Bandnummer96

Heftnummer2

ISSN0007-4861

eISSN1432-0800

DOI Linkhttps://doi.org/10.1007/s00128-015-1699-x

VerlagSpringer


Abstract
Marking of fish otoliths with oxytetracycline and tetracycline is a widely used method to evaluate the effectiveness of stocking operations. Available protocols for the labeling of fish specify a number of factors influencing mark quality and potential risk for fish during marking. This study investigates the influence of water hardness on mortality of freshwater fish during marking with OTC. In order to pursue this question complexation of OTC with Mg2+ and Ca2+ cations was measured spectrophotometrically. Furthermore, zebrafish (Danio rerio) were immersed in OTC solutions (1200 mg/L; 48 h immersion) combined with varying levels of water hardness (5.5, 15.5, 25.5, 32.5A degrees dH). The amount of OTC-Mg-Ca-complexes was positively correlated to water hardness. Moreover, it could be demonstrated that mortality of zebrafish during marking varied as a factor of water hardness. Highest mortalities occurred at the lowest (5.5A degrees dH) and the highest (32.5A degrees dH) tested levels during marking with OTC.



Zitierstile

Harvard-ZitierstilHundt, M., Schreiber, B., Eckmann, R., Lunestad, B., Wuenneman, H. and Schulz, R. (2016) The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline, Bulletin of Environmental Contamination and Toxicology, 96(2), pp. 144-149. https://doi.org/10.1007/s00128-015-1699-x

APA-ZitierstilHundt, M., Schreiber, B., Eckmann, R., Lunestad, B., Wuenneman, H., & Schulz, R. (2016). The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline. Bulletin of Environmental Contamination and Toxicology. 96(2), 144-149. https://doi.org/10.1007/s00128-015-1699-x



Schlagwörter


BROWN TROUTDanio rerioFINGERLINGSFIN SPINESGILLSImmersion markingMARKINGOTOLITHSRestockingWater hardness


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