Journalartikel

Jahr der Veröffentlichung: 2014

Seiten: 839-846

Zeitschrift: Journal of Radiation Oncology, Biology, Physics

Bandnummer: 190

Heftnummer: 9

ISSN: 0179-7158

eISSN: 1439-099X

DOI Link: https://doi.org/10.1007/s00066-014-0605-5

Verlag: Springer

Abstract: 

Background and purpose. Human Papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) respond favourably to radiotherapy as compared to HPV-unrelated HNSCC. We investigated DNA damage response in HPV-positive and HPV-negative HNSCC cell lines aiming to identify mechanisms, which illustrate reasons for the increased sensitivity of HPV-positive cancers of the oropharynx.

Methods. Radiation response including clonogenic survival, apoptosis, DNA double-strand break (DSB) repair, and cell cycle redistribution in four HPV-positive (UM-SCC-47, UM-SCC-104, 93-VU-147T, UPCI:SCC152) and four HPV-negative (UD-SCC-1, UM-SCC-6, UM-SCC-11b, UT-SCC-33) cell lines was evaluated.

Results. HPV-positive cells were more radiosensitive (mean SF2: 0.198 range: 0.22-0.18) than HPV-negative cells (mean SF2: 0.34, range: 0.45-0.27; p = 0.010). Irradiated HPV-positive cell lines progressed faster through S-phase showing a more distinct accumulation in G2/M. The abnormal cell cycle checkpoint activation was accompanied by a more pronounced increase of cell death after x-irradiation and a higher number of residual and unreleased DSBs.

Conclusions. The enhanced responsiveness of HPV-related HNSCC to radiotherapy might be caused by a higher cellular radiosensitivity due to cell cycle dysregulation and impaired DNA DSB repair.

Harvard-Zitierstil: Arenz, A., Ziemann, F., Mayer, C., Wittig, A., Dreffke, K., Preising, S., et al. (2014) Increased radiosensitivity of HPV-positive head and neck cancer cell lines due to cell cycle dysregulation and induction of apoptosis, Journal of Radiation Oncology, Biology, Physics, 190(9), pp. 839-846. https://doi.org/10.1007/s00066-014-0605-5

APA-Zitierstil: Arenz, A., Ziemann, F., Mayer, C., Wittig, A., Dreffke, K., Preising, S., Wagner, S., Klussmann, J., Engenhart-Cabillic, R., & Wittekindt, C. (2014). Increased radiosensitivity of HPV-positive head and neck cancer cell lines due to cell cycle dysregulation and induction of apoptosis. Journal of Radiation Oncology, Biology, Physics. 190(9), 839-846. https://doi.org/10.1007/s00066-014-0605-5