Journalartikel
Autorenliste: Abdullah, Zeinab; Schlee, Martin; Roth, Susanne; Abu Mraheil, Mobarak; Barchet, Winfried; Boettcher, Jan; Hain, Torsten; Geiger, Sergej; Hayakawa, Yoshihiro; Fritz, Joerg H.; Civril, Filiz; Hopfner, Karl-Peter; Kurts, Christian; Ruland, Juergen; Hartmann, Gunther; Chakraborty, Trinad; Knolle, Percy A.
Jahr der Veröffentlichung: 2012
Seiten: 4153-4164
Zeitschrift: The EMBO Journal
Bandnummer: 31
Heftnummer: 21
ISSN: 0261-4189
eISSN: 1460-2075
Open Access Status: Green
DOI Link: https://doi.org/10.1038/emboj.2012.274
Verlag: EMBO Press
Abstract:
Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria to the cytoplasm of infected cells. We found that Listeria released nucleic acids and that such secreted bacterial RNA/DNA was recognized by the cytosolic sensors RIG-I, MDA5 and STING thereby triggering interferon beta production. Secreted Listeria nucleic acids also caused RIG-I-dependent IL-1 beta-production and inflammasome activation. The signalling molecule CARD9 contributed to IL-1 beta production in response to secreted nucleic acids. In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG-I provides a mechanistic explanation for efficient induction of immunity by live bacteria. The EMBO Journal (2012) 31, 4153-4164. doi: 10.1038/emboj.2012.274; Published online 12 October 2012
Zitierstile
Harvard-Zitierstil: Abdullah, Z., Schlee, M., Roth, S., Abu Mraheil, M., Barchet, W., Boettcher, J., et al. (2012) RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids, The EMBO Journal, 31(21), pp. 4153-4164. https://doi.org/10.1038/emboj.2012.274
APA-Zitierstil: Abdullah, Z., Schlee, M., Roth, S., Abu Mraheil, M., Barchet, W., Boettcher, J., Hain, T., Geiger, S., Hayakawa, Y., Fritz, J., Civril, F., Hopfner, K., Kurts, C., Ruland, J., Hartmann, G., Chakraborty, T., & Knolle, P. (2012). RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids. The EMBO Journal. 31(21), 4153-4164. https://doi.org/10.1038/emboj.2012.274
Schlagwörter
AIM2 INFLAMMASOME; INFLAMMASOME ACTIVATION; Listeria infection; MONOCYTOGENES INFECTION; nucleic-acid secretion; PROTECTIVE IMMUNITY
