Journal article
Authors list: Abdullah, Zeinab; Schlee, Martin; Roth, Susanne; Abu Mraheil, Mobarak; Barchet, Winfried; Boettcher, Jan; Hain, Torsten; Geiger, Sergej; Hayakawa, Yoshihiro; Fritz, Joerg H.; Civril, Filiz; Hopfner, Karl-Peter; Kurts, Christian; Ruland, Juergen; Hartmann, Gunther; Chakraborty, Trinad; Knolle, Percy A.
Publication year: 2012
Pages: 4153-4164
Journal: The EMBO Journal
Volume number: 31
Issue number: 21
ISSN: 0261-4189
eISSN: 1460-2075
Open access status: Green
DOI Link: https://doi.org/10.1038/emboj.2012.274
Publisher: EMBO Press
Abstract:
Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria to the cytoplasm of infected cells. We found that Listeria released nucleic acids and that such secreted bacterial RNA/DNA was recognized by the cytosolic sensors RIG-I, MDA5 and STING thereby triggering interferon beta production. Secreted Listeria nucleic acids also caused RIG-I-dependent IL-1 beta-production and inflammasome activation. The signalling molecule CARD9 contributed to IL-1 beta production in response to secreted nucleic acids. In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG-I provides a mechanistic explanation for efficient induction of immunity by live bacteria. The EMBO Journal (2012) 31, 4153-4164. doi: 10.1038/emboj.2012.274; Published online 12 October 2012
Citation Styles
Harvard Citation style: Abdullah, Z., Schlee, M., Roth, S., Abu Mraheil, M., Barchet, W., Boettcher, J., et al. (2012) RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids, The EMBO Journal, 31(21), pp. 4153-4164. https://doi.org/10.1038/emboj.2012.274
APA Citation style: Abdullah, Z., Schlee, M., Roth, S., Abu Mraheil, M., Barchet, W., Boettcher, J., Hain, T., Geiger, S., Hayakawa, Y., Fritz, J., Civril, F., Hopfner, K., Kurts, C., Ruland, J., Hartmann, G., Chakraborty, T., & Knolle, P. (2012). RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids. The EMBO Journal. 31(21), 4153-4164. https://doi.org/10.1038/emboj.2012.274
Keywords
AIM2 INFLAMMASOME; INFLAMMASOME ACTIVATION; Listeria infection; MONOCYTOGENES INFECTION; nucleic-acid secretion; PROTECTIVE IMMUNITY
