Journal article
Authors list: Kalekar, S.; Rodrigues, J.; D'Costa, D.; Doijad, S.; Kumar, J. Ashok; Malik, S. V. S.; Kalorey, D. R.; Rawool, D. B.; Hain, T.; Chakraborty, T.; Barbuddhe, S. B.
Publication year: 2011
Pages: 351-358
Journal: Annals of Tropical Medicine and Parasitology
Volume number: 105
Issue number: 5
ISSN: 0003-4983
eISSN: 1364-8594
Open access status: Green
DOI Link: https://doi.org/10.1179/1364859411Y.0000000023
Publisher: Taylor and Francis
Abstract:
Listeria monocytogenes is a foodborne pathogen associated with severe diseases in humans and animals. The genotypic analysis of 17 L. monocytogenes isolates recovered from humans in India during 2006-2009 using multiplex serotyping PCR allowing serovar predictions, conventional serology and by pulsed field gel electrophoresis (PFGE) is presented. The isolates were recovered from patients exhibiting various clinical conditions. A multiplex-PCR based serotyping assay revealed 88.24% (15/17) of the strains belonging to the serovar group 4b, 4d, 4e and 11.76% (2/17) to the serovar group 1/2b, 3b. Conventional serology indicated that 13 (76.47%) L. monocytogenes isolates to be of serotype 4b, 2 (11.76%) serotype 4d, and 2 (11.76%) serotype 1/2b. Ten ApaI and nine AscI pulsotypes were recognized among the 17 human isolates. PFGE analysis allowed discrimination among isolates of the same serotype and among isolates from the same sampling areas or those isolated from different areas. Thus, PFGE together with multiplex-PCR serotyping allows rapid discrimination of L. monocytogenes strains. In addition, the predominance of L. monocytogenes serotype 4b is of concern, as this serotype has been most frequently associated with human listeriosis outbreaks.
Citation Styles
Harvard Citation style: Kalekar, S., Rodrigues, J., D'Costa, D., Doijad, S., Kumar, J., Malik, S., et al. (2011) Genotypic characterization of Listeria monocytogenes isolated from humans in India, Annals of Tropical Medicine and Parasitology, 105(5), pp. 351-358. https://doi.org/10.1179/1364859411Y.0000000023
APA Citation style: Kalekar, S., Rodrigues, J., D'Costa, D., Doijad, S., Kumar, J., Malik, S., Kalorey, D., Rawool, D., Hain, T., Chakraborty, T., & Barbuddhe, S. (2011). Genotypic characterization of Listeria monocytogenes isolated from humans in India. Annals of Tropical Medicine and Parasitology. 105(5), 351-358. https://doi.org/10.1179/1364859411Y.0000000023
Keywords
SPP.
