Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 167-177

Zeitschrift: Amino Acids

Bandnummer: 38

Heftnummer: 1

ISSN: 0939-4451

eISSN: 1438-2199

DOI Link: https://doi.org/10.1007/s00726-008-0224-5

Verlag: Springer

Abstract: 
The aim of this study was to determine the effects of alpha-ketoglutarate on neutrophil (PMN), free alpha-keto and amino-acid profiles as well as important reactive oxygen species (ROS) produced [superoxide anion (O-2(-)), hydrogen peroxide (H2O2)] and released myeloperoxidase (MPO) acitivity. Exogenous alpha-ketoglutarate significantly increased PMN alpha-ketoglutarate, pyruvate, asparagine, glutamine, asparatate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Additionally, in parallel with intracellular alpha-ketoglutarate changes, increases in O-2(-) formation, H2O2-generation and MPO acitivity have also been observed. We therefore believe that alpha-ketoglutarate is important for affecting PMN "susceptible free amino- and alpha-keto acid pools" although important mechanisms and backgrounds are not yet completely explored. Moreover, our results also show very clearly that changes in intragranulocytic alpha-ketoglutarate levels are relevant metabolic determinants in PMN nutrition considerably influencing and modulating the magnitude and quality of the granulocytic host defense capability as well as production of ROS.

Harvard-Zitierstil: Muehling, J., Tussing, F., Nickolaus, K., Matejec, R., Henrich, M., Harbach, H., et al. (2010) Effects of α-ketoglutarate on neutrophil intracellular amino and α-keto acid profiles and ROS production, Amino Acids, 38(1), pp. 167-177. https://doi.org/10.1007/s00726-008-0224-5

APA-Zitierstil: Muehling, J., Tussing, F., Nickolaus, K., Matejec, R., Henrich, M., Harbach, H., Wolff, M., Weismueller, K., Engel, J., Welters, I., Langefeld, T., Fuchs, M., Weigand, M., & Heidt, M. (2010). Effects of α-ketoglutarate on neutrophil intracellular amino and α-keto acid profiles and ROS production. Amino Acids. 38(1), 167-177. https://doi.org/10.1007/s00726-008-0224-5