Journal article

Publication year: 2010

Pages: 167-177

Journal: Amino Acids

Volume number: 38

Issue number: 1

ISSN: 0939-4451

eISSN: 1438-2199

DOI Link: https://doi.org/10.1007/s00726-008-0224-5

Publisher: Springer

Abstract: 
The aim of this study was to determine the effects of alpha-ketoglutarate on neutrophil (PMN), free alpha-keto and amino-acid profiles as well as important reactive oxygen species (ROS) produced [superoxide anion (O-2(-)), hydrogen peroxide (H2O2)] and released myeloperoxidase (MPO) acitivity. Exogenous alpha-ketoglutarate significantly increased PMN alpha-ketoglutarate, pyruvate, asparagine, glutamine, asparatate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Additionally, in parallel with intracellular alpha-ketoglutarate changes, increases in O-2(-) formation, H2O2-generation and MPO acitivity have also been observed. We therefore believe that alpha-ketoglutarate is important for affecting PMN "susceptible free amino- and alpha-keto acid pools" although important mechanisms and backgrounds are not yet completely explored. Moreover, our results also show very clearly that changes in intragranulocytic alpha-ketoglutarate levels are relevant metabolic determinants in PMN nutrition considerably influencing and modulating the magnitude and quality of the granulocytic host defense capability as well as production of ROS.

Harvard Citation style: Muehling, J., Tussing, F., Nickolaus, K., Matejec, R., Henrich, M., Harbach, H., et al. (2010) Effects of α-ketoglutarate on neutrophil intracellular amino and α-keto acid profiles and ROS production, Amino Acids, 38(1), pp. 167-177. https://doi.org/10.1007/s00726-008-0224-5

APA Citation style: Muehling, J., Tussing, F., Nickolaus, K., Matejec, R., Henrich, M., Harbach, H., Wolff, M., Weismueller, K., Engel, J., Welters, I., Langefeld, T., Fuchs, M., Weigand, M., & Heidt, M. (2010). Effects of α-ketoglutarate on neutrophil intracellular amino and α-keto acid profiles and ROS production. Amino Acids. 38(1), 167-177. https://doi.org/10.1007/s00726-008-0224-5