Journalartikel

GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR


AutorenlisteMOSGES, G; FRIEDT, W

Jahr der Veröffentlichung1994

Seiten114-124

ZeitschriftPlant Breeding

Bandnummer113

Heftnummer2

ISSN0179-9541

DOI Linkhttps://doi.org/10.1111/j.1439-0523.1994.tb00713.x

VerlagWiley


Abstract
Three different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybrids were analysed by three isozymes, oligonucleotides (GACA)(4), (GATA)(4) and (GGAT)(4) as hybridization probes, and 70 primers for PCR analysis. Polymorphisms were visible with each type of marker. However, the repeated probes, especially (GATA)(4), revealed the highest degree of variability and seem to be most appropriate for the generation of genotype-specific 'fingerprints.'



Zitierstile

Harvard-ZitierstilMOSGES, G. and FRIEDT, W. (1994) GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR, Plant Breeding, 113(2), pp. 114-124. https://doi.org/10.1111/j.1439-0523.1994.tb00713.x

APA-ZitierstilMOSGES, G., & FRIEDT, W. (1994). GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR. Plant Breeding. 113(2), 114-124. https://doi.org/10.1111/j.1439-0523.1994.tb00713.x



Schlagwörter


AMPLIFIED POLYMORPHIC DNAAP-PCRARBITRARY PRIMERSDNA FINGERPRINTINGGENOMESHELIANTHUS ANNUUSISOZYMESMARKERSMINISATELLITESRAPD

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