Journalartikel

Jahr der Veröffentlichung: 1982

Seiten: 929-939

Zeitschrift: Bioscience Reports

Bandnummer: 2

Heftnummer: 11

ISSN: 0144-8463

eISSN: 1573-4935

DOI Link: https://doi.org/10.1007/BF01114900

Verlag: Portland Press

Abstract: 
The RNA genome of potato spindle tuber viroid (PSTV) is transcribed in vitro into complementary DNA and RNA by DNA-dependent DNA polymeraes I and RNA polymerase, respectively, from E. coli. In vitro synthesis of complementary RNA produces distinct transcripts larger than unit length thus reflecting the in vivo mechanism of viroid replication. The influence of varying experimental conditions on the transcription process is studied; actinomycin D is found to drastically reduce complementary RNA synthesis from the PSTV RNA template by RNA polymerase.

Harvard-Zitierstil: ROHDE, W., RACKWITZ, H., BOEGE, F. and SANGER, H. (1982) VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI, Bioscience Reports, 2(11), pp. 929-939. https://doi.org/10.1007/BF01114900

APA-Zitierstil: ROHDE, W., RACKWITZ, H., BOEGE, F., & SANGER, H. (1982). VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI. Bioscience Reports. 2(11), 929-939. https://doi.org/10.1007/BF01114900