VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI - Research portal of Justus Liebig University Giessen:

Journal article

VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI

Authors list: ROHDE, W; RACKWITZ, HR; BOEGE, F; SANGER, HL

Publication year: 1982

Pages: 929-939

Journal: Bioscience Reports

Volume number: 2

Issue number: 11

ISSN: 0144-8463

eISSN: 1573-4935

DOI Link: https://doi.org/10.1007/BF01114900

Publisher: Portland Press

Abstract:
The RNA genome of potato spindle tuber viroid (PSTV) is transcribed in vitro into complementary DNA and RNA by DNA-dependent DNA polymeraes I and RNA polymerase, respectively, from E. coli. In vitro synthesis of complementary RNA produces distinct transcripts larger than unit length thus reflecting the in vivo mechanism of viroid replication. The influence of varying experimental conditions on the transcription process is studied; actinomycin D is found to drastically reduce complementary RNA synthesis from the PSTV RNA template by RNA polymerase.

Citation Styles

Harvard Citation style: ROHDE, W., RACKWITZ, H., BOEGE, F. and SANGER, H. (1982) VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI, Bioscience Reports, 2(11), pp. 929-939. https://doi.org/10.1007/BF01114900

APA Citation style: ROHDE, W., RACKWITZ, H., BOEGE, F., & SANGER, H. (1982). VIROID RNA IS ACCEPTED AS A TEMPLATE FOR INVITRO TRANSCRIPTION BY DNA-DEPENDENT DNA-POLYMERASE-I AND RNA-POLYMERASE FROM ESCHERICHIA-COLI. Bioscience Reports. 2(11), 929-939. https://doi.org/10.1007/BF01114900

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