Journalartikel

Jahr der Veröffentlichung: 1979

Seiten: 141-143

Zeitschrift: Clinical Chemistry

Bandnummer: 25

Heftnummer: 1

ISSN: 0009-9147

eISSN: 1530-8561

Verlag: Oxford University Press

Abstract: 
This paper deals with removal of contaminants before gas-chromatographic determination of aldosterone in [human] urine. Urine is incubated with .beta.-glucuronidase [Escherichia coli], which hydrolyzes all .beta.-glucuronides except aldosterone-18-glucuronide. The contaminants (the aglycones released and other methylene chloride-soluble substances) are extracted with methylene chloride. Solvolysis of the aqueous phase liberates aldosterone from aldosterone-18-glucuronide, which then is extracted with methylene chloride and oxidized by use of periodic acid. The resulting lactone can easily be separated by 1-dimensional TLC and determined by GLC.

Harvard-Zitierstil: GRAEF, V., FURUYA, E. and NISHIKAZE, O. (1979) REMOVAL OF CONTAMINATING SUBSTANCES BEFORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALDOSTERONE IN URINE, Clinical Chemistry, 25(1), pp. 141-143

APA-Zitierstil: GRAEF, V., FURUYA, E., & NISHIKAZE, O. (1979). REMOVAL OF CONTAMINATING SUBSTANCES BEFORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALDOSTERONE IN URINE. Clinical Chemistry. 25(1), 141-143.