Journal article
Authors list: GRAEF, V; FURUYA, E; NISHIKAZE, O
Publication year: 1979
Pages: 141-143
Journal: Clinical Chemistry
Volume number: 25
Issue number: 1
ISSN: 0009-9147
eISSN: 1530-8561
Publisher: Oxford University Press
Abstract:
This paper deals with removal of contaminants before gas-chromatographic determination of aldosterone in [human] urine. Urine is incubated with .beta.-glucuronidase [Escherichia coli], which hydrolyzes all .beta.-glucuronides except aldosterone-18-glucuronide. The contaminants (the aglycones released and other methylene chloride-soluble substances) are extracted with methylene chloride. Solvolysis of the aqueous phase liberates aldosterone from aldosterone-18-glucuronide, which then is extracted with methylene chloride and oxidized by use of periodic acid. The resulting lactone can easily be separated by 1-dimensional TLC and determined by GLC.
Citation Styles
Harvard Citation style: GRAEF, V., FURUYA, E. and NISHIKAZE, O. (1979) REMOVAL OF CONTAMINATING SUBSTANCES BEFORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALDOSTERONE IN URINE, Clinical Chemistry, 25(1), pp. 141-143
APA Citation style: GRAEF, V., FURUYA, E., & NISHIKAZE, O. (1979). REMOVAL OF CONTAMINATING SUBSTANCES BEFORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALDOSTERONE IN URINE. Clinical Chemistry. 25(1), 141-143.
