Journal article

Publication year: 1978

Pages: 7-12

Journal: Planta

Volume number: 144

Issue number: 1

ISSN: 0032-0935

eISSN: 1432-2048

DOI Link: https://doi.org/10.1007/BF00385001

Publisher: Springer

Abstract: 
Using a 14C/3H double-labeling technique, the influence of kinetin on the length of the cell cycle of meristematic cells in haploid and diploid callus cultures of D. innoxia was determined. The total length of the cell cycle of haploid cells as compared to that of diploid cells was reduced by 2.3 h (-kinetin) or 1.4 h (+kinetin). The addition of kinetin to the nutrient solution also reduces cell cycle duration at both ploidy levels. For synchronization of the cell cycle, a fluorodesoxyuridine/thymidine system was successfully employed. Apparently, the reduction of total cell cycle duration of cycling cells due to treatment with kinetin occurred at the expense of the G1 phase. Nevertheless, kinetic seems to exert an influence on the transition of cells from the G2 into the M phase as well.

Harvard Citation style: BLASCHKE, J., FORCHE, E. and NEUMANN, K. (1978) INVESTIGATIONS ON CELL-CYCLE OF HAPLOID AND DIPLOID TISSUE-CULTURES OF DATURA-INNOXIA MILL AND ITS SYNCHRONIZATION, Planta, 144(1), pp. 7-12. https://doi.org/10.1007/BF00385001

APA Citation style: BLASCHKE, J., FORCHE, E., & NEUMANN, K. (1978). INVESTIGATIONS ON CELL-CYCLE OF HAPLOID AND DIPLOID TISSUE-CULTURES OF DATURA-INNOXIA MILL AND ITS SYNCHRONIZATION. Planta. 144(1), 7-12. https://doi.org/10.1007/BF00385001