Journalartikel

Jahr der Veröffentlichung: 2024

Zeitschrift: STAR Protocols

Bandnummer: 5

Heftnummer: 3

ISSN: 2666-1667

DOI Link: https://doi.org/10.1016/j.xpro.2024.103248

Verlag: Cell Press

Abstract: 
The spotted wing Drosophila ( Drosophila suzukii Matsumura) is recognized globally as a significant economic pest. Here, we present a protocol for genetic engineering in D. suzukii using microinjection. We describe steps for genetic engineering techniques, including transposon-mediated germline transformation, recombinase-mediated genome targeting, and CRISPR-mediated gene editing. This protocol can significantly expand the toolkit for functional genomics and genetic control studies of this pest. For complete details on the use and execution of this protocol, please refer to Schetelig and Handler,1 Schetelig et al. 2 Yan et al.,3 and Yan et al.4

Harvard-Zitierstil: Yan, Y. and Schetelig, M. (2024) Protocol for genetic engineering in Drosophila suzukii using microinjection, STAR Protocols, 5(3), Article 103248. https://doi.org/10.1016/j.xpro.2024.103248

APA-Zitierstil: Yan, Y., & Schetelig, M. (2024). Protocol for genetic engineering in Drosophila suzukii using microinjection. STAR Protocols. 5(3), Article 103248. https://doi.org/10.1016/j.xpro.2024.103248