Journal article

Publication year: 2024

Journal: STAR Protocols

Volume number: 5

Issue number: 3

ISSN: 2666-1667

DOI Link: https://doi.org/10.1016/j.xpro.2024.103248

Publisher: Cell Press

Abstract: 
The spotted wing Drosophila ( Drosophila suzukii Matsumura) is recognized globally as a significant economic pest. Here, we present a protocol for genetic engineering in D. suzukii using microinjection. We describe steps for genetic engineering techniques, including transposon-mediated germline transformation, recombinase-mediated genome targeting, and CRISPR-mediated gene editing. This protocol can significantly expand the toolkit for functional genomics and genetic control studies of this pest. For complete details on the use and execution of this protocol, please refer to Schetelig and Handler,1 Schetelig et al. 2 Yan et al.,3 and Yan et al.4

Harvard Citation style: Yan, Y. and Schetelig, M. (2024) Protocol for genetic engineering in Drosophila suzukii using microinjection, STAR Protocols, 5(3), Article 103248. https://doi.org/10.1016/j.xpro.2024.103248

APA Citation style: Yan, Y., & Schetelig, M. (2024). Protocol for genetic engineering in Drosophila suzukii using microinjection. STAR Protocols. 5(3), Article 103248. https://doi.org/10.1016/j.xpro.2024.103248