Protein-chemical standardization of the erythrocyte glutathione reductase activation test (EGRAC test) - Application to hypothyroidism - Research portal of Justus Liebig University Giessen:

Journal article

Protein-chemical standardization of the erythrocyte glutathione reductase activation test (EGRAC test) - Application to hypothyroidism

Authors list: Becker, K; Krebs, B; Schirmer, RH

Publication year: 1991

Pages: 180-187

Journal: International Journal for Vitamin and Nutrition Research

Volume number: 61

Issue number: 2

ISSN: 0300-9831

eISSN: 1664-2821

Publisher: Hogrefe

Abstract:
The erythrocyte glutathione reductase activation coefficient (EGRAC) is an index of riboflavin deficiency or, more exactly, of FAD deficiency in man. In this report a sensitive version of the EGRAC test is introduced that is based on the molecular properties of glutathione reductase and its FAD-free apoenzyme. The hemoglobin concentration of the blood sample can be estimated simultaneously using the spectrophotometric absorption at 340 nm. - The method was tested for 33 thyroidectomized patients in comparison with a euthyroid control group. From the average EGRAC values (1.40 vs. 1.22) it was deduced that the average free FAD level was approximately 2 times lower for the patients' than for the control group. Discussed is the role of the EGRAC test in hormonal and nutritional disorders.

Citation Styles

Harvard Citation style: Becker, K., Krebs, B. and Schirmer, R. (1991) Protein-chemical standardization of the erythrocyte glutathione reductase activation test (EGRAC test) - Application to hypothyroidism, International Journal for Vitamin and Nutrition Research, 61(2), pp. 180-187

APA Citation style: Becker, K., Krebs, B., & Schirmer, R. (1991). Protein-chemical standardization of the erythrocyte glutathione reductase activation test (EGRAC test) - Application to hypothyroidism. International Journal for Vitamin and Nutrition Research. 61(2), 180-187.