Journalartikel

Jahr der Veröffentlichung: 2007

Seiten: 118-125

Zeitschrift: Inflammation Research

Bandnummer: 56

Heftnummer: 3

ISSN: 1023-3830

DOI Link: https://doi.org/10.1007/s00011-006-6122-y

Verlag: Springer

Abstract: 
Objective and design: The effect of oxidized frying oils on PPAR gamma which is a potent inhibitor of inflammatory responses in the intestine is currently unknown. Thus, the present study aimed to explore the effect of oxidized frying oil on PPAR gamma DNA-binding and proinflammatory responses in intestinal epithelial cells.Material and methods: 18 male pigs were fed two different diets containing either fresh fat or oxidized fat (n = 9 each). After 28 d, intestinal epithelial cells were isolated and analyzed for PPAR gamma DNA-binding, NF-kappa B DNA-binding and NF-kappa B target gene expression. In addition, markers of lipid peroxidation and antioxidant status were determined.Results: Feeding the oxidized fat slightly, but not significantly, activated PPAR gamma DNA-binding in intestinal epithelial cells when compared to fresh fat. In addition, oxidized fat increased the concentration of TBARS and reduced the concentrations of cc-tocopherol and activities of antioxidant enzymes relative to fresh fat (P < 0.05). No effect of the oxidized fat was observed on NF-kappa B DNA-binding and NF-kappa B target gene expression in intestinal epithelial cells.Conclusions: The present study suggests that moderate PPAR gamma activation and induction of oxidative stress by oxidized frying oil have no implication for NF-kappa B-mediated proinflammatory gene expression in porcine intestinal epithelial cells.

Harvard-Zitierstil: Ringseis, R., Piwek, N. and Eder, K. (2007) Oxidized fat induces oxidative stress but has no effect on NF-kappa B-mediated proinflammatory gene transcription in porcine intestinal epithelial cells, Inflammation Research, 56(3), pp. 118-125. https://doi.org/10.1007/s00011-006-6122-y

APA-Zitierstil: Ringseis, R., Piwek, N., & Eder, K. (2007). Oxidized fat induces oxidative stress but has no effect on NF-kappa B-mediated proinflammatory gene transcription in porcine intestinal epithelial cells. Inflammation Research. 56(3), 118-125. https://doi.org/10.1007/s00011-006-6122-y