Journalartikel

Jahr der Veröffentlichung: 2021

Zeitschrift: Journal of Chromatography A

Bandnummer: 1635

ISSN: 0021-9673

eISSN: 1873-3778

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1016/j.chroma.2020.461727

Verlag: Elsevier

Abstract: 

Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyland butyrylcholinesterases, alpha- and beta-glucosidases and alpha-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms.

Harvard-Zitierstil: Moricz, A., Krüzselyi, D., Ott, P., Garadi, Z., Beni, S., Morlock, G., et al. (2021) Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract, Journal of Chromatography A, 1635, Article 461727. https://doi.org/10.1016/j.chroma.2020.461727

APA-Zitierstil: Moricz, A., Krüzselyi, D., Ott, P., Garadi, Z., Beni, S., Morlock, G., & Bakonyi, J. (2021). Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract. Journal of Chromatography A. 1635, Article 461727. https://doi.org/10.1016/j.chroma.2020.461727